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| Brilhante,Andreia Fernandes; Dorval,Maria Elizabeth Cavalheiros; Galati,Eunice Aparecida Bianchi; Castro,Vivianne de Oliveira Landgraf de; Rocca,Maria Elizabeth Ghizzi; Facco,Gilberto Gonçalves; Souza,Celeste da Silva Freitas de; Nunes,Vânia Lúcia Brandão. |
| ABSTRACT: The study aimed to investigate Leishmania infection in a population of dogs (92 animals) in a fishing area of Bonito Municipality, and evaluate comparatively the serological methods used, immunoenzymatic assay (ELISA), indirect immunofluorescence antibody test (IFAT), and rapid Dual Path-Platform test (DPP®). Blood and aspirate of bone marrow samples were used and parasitological investigation was also performed, such as parasite isolation in Neal, Novy, Nicolle (NNN) medium culture, Woo technique, Giemsa stained smears and specific identification by polymerase chain reaction (PCR). IFAT revealed 56/92 seropositive, the ELISA 8/92 and the DPP®41/92. Regarding the agreement between the serological tests by the Kappa index, there was a slight... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Domestic dogs; Serodiagnosis; Ecotourism. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000300501 |
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